A total of 95 bacteria from six different epidemiologically important multilocus sequence types ST10, ST69, ST95, ST73, ST127 and ST131 were used in this project and principal component-discriminant function analysis PC-DFA of these samples produced clear separate clustering of isolates, based on the ST
Analysis of data using partial least squares-discriminant analysis PLS-DA , incorporating cross-validation, indicated a high prediction accuracy of 91 With this micro-culture approach we also demonstrate high reproducibility of the metabolic fingerprints

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However, whilst FT-IR spectroscopy is fast typically 10 s to 1 min per sample , the approaches for microbial sample preparation can be time consuming as plate culture or shake flasks are used for growth of the organism.

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These results suggest that FT-IR spectroscopy could be a useful method for the rapid identification of members of important UPEC STs
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The approach allowed separation of different isolates of Escherichia coli involved in urinary tract infection, including members of the globally disseminated ST131 clone, with respect to both genotype and resistance or otherwise to the antibiotic Ciprofloxacin
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coli reveal different information content in terms of metabolic potential compared to virulence factors

هيثم بن خالد محمد الربيعة

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